Methods for protein binding enzyme complementation assays
US4708929A · kind A · utility
71Cited by
29References
4Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Apr 8, 1985 |
| Grant date | Nov 24, 1987 |
| Priority date | — |
| Expiry date | Apr 8, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.