Preparation of secretin

US4711847A · kind A · utility

Assignee

• 501 Hoechst Aktiengesellschaft · DE

Inventors

• Wolfgang Konig · Hofheim, DE
• Joachim Engels · Kronberg, DE
• Eugen Uhlmann · Königstein, DE
• Waldemar Wetekam · Eppstein, DE

Key dates

• Filing date: Aug 8, 1984
• Grant date: Dec 8, 1987
• Expiry date: Aug 8, 2004

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/75
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Secretin, which cannot be prepared directly by genetic engineering because of its carboxylic acid carboxyl-terminus, can be obtained by preparing secretylglycine by genetic engineering and then obtaining secretin therefrom by enzymatic conversion of the terminal glycine radical. The gene for the secretylglycine is synthesized chemically from smaller single-stranded units which are linked enzymatically to give the complete gene, incorporated into a suitable vector and amplified therein, after which the peptide is isolated directly or as a fusion protein and, after cyanogen bromide cleavage, is converted enzymatically into secretin.