Templet for simultaneous screening of several antibodies and method of using the same
US4713349A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 25, 1985 |
| Grant date | Dec 15, 1987 |
| Priority date | — |
| Expiry date | Feb 25, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/809
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A templet (10) is provided having a lower plate (11) which receives a carrier sheet having antigens immobilized thereon as transferred from an electrophoresis gel. An upper plate (12) having a plurality of parallel channels (22) formed therein, is placed over the lower plate such that the channels lie over the carrier sheet. The two plates (11, 12) are then pressed tightly together, as by engagement with screws (16) at the periphery of the plates. A bridge bar (28) extends over the central portion of the plate and pressure screws (30) are mounted to the bridge bar in position to engage and apply pressure to the top surface of the upper plate (12). Various antibody containing liquids are introduced into the various channels through holes (25) extending to the ends of the channels; the solutions are allowed to remain in contact with the antigens on the carrier sheet for a period of time, after which the liquid is withdrawn from the channels, and the plates separated. The entire carrier sheet is subsequently exposed to tracer antibodies labeled radioactively or otherwise. The carrier sheet so treated shows a series of parallel vertical stripes, in each of which those horizontal antige…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.