Rapid enzyme assay by product selective blot
US4716101A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 18, 1984 |
| Grant date | Dec 29, 1987 |
| Priority date | — |
| Expiry date | Dec 18, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and assay kit for determining the presence of an enzyme in a sample are provided. A sample suspected of containing the enzyme is applied to an image gel, which may be any conventional material, typically agarose gel. The sample may first be subjected to electrophoresis, or may be detected directly using the present invention. The image gel includes an immobilized phase capable of binding a product of the enzyme but not the substrate. By exposing the enzyme to substrate, and drawing the resulting reaction mixtures through the image gel, only the product is bound in the image gel. The presence of enzyme may then be determined by detecting the product in the image gel. In addition to developing electrophoresis gels, the method of the present invention will find great use in screening a plurality of complex mixtures which have been separated by other conventional techniques.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.