Solid phase biological diagnostic assay via visual observation as enhanced by Mie scattering
US4716123A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 20, 1986 |
| Grant date | Dec 29, 1987 |
| Priority date | — |
| Expiry date | Aug 20, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and kits are set out having improved selectivity and improved sensitivity for use in field and home immunoassays. A specifically binding biomaterial is attached to a macroextensive surface of a plastic strip or the like. A biological substance which is a specific binding partner to a binding site of the specifically binding biomaterial is attached to each of a plurality of synthetic particles. The particles are of a preselected size, refractive index, or the like to enhance their visibility in accordance with the Mie scattering phenomenon. Testing is by either contacting the particles with the strips to obtain adherence of the particles to the strips, or by exposing strips having the particles already adhering to them to a solution containing either the specifically binding biomaterial or the biological substance, whereby the particles adherence to the strip is eliminated. A quick and accurate pregnancy test is one result of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.