Detection of bacteria by fluorescent staining in an expanded buffy coat
US4717660A · kind A · utility
29Cited by
6References
17Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jan 26, 1984 |
| Grant date | Jan 5, 1988 |
| Priority date | — |
| Expiry date | Jan 26, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2304/12
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the detection of microorganisms e.g. bacteria in a blood sample comprises staining the microorganisms with a fluorescent dye and observing the fluorescence emission of an expanded buffy coat. The expanded buffy coat is obtained by centrifuging the stained sample in a hematocrit tube containing a float which occupies most of the buffy coat volume. The expanded buffy coat further separates into sub-layers. Intracellular microorganisms congregate in and are detected in the granulocyte sub-layer. The method may be modified to assess phagocytic activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.