Method for producing secondary metabolites of plants
US4717664A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 16, 1985 |
| Grant date | Jan 5, 1988 |
| Priority date | — |
| Expiry date | Aug 16, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/91
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides for a method for producing secondary metabolites of higher plants, such as Lithospermum erythrorhizon, Coptis japonica, and Nicotinia tabacum, by suspension cultures of a mass of undifferentiated cells (callus). The cultures are carried out in at least two stages in liquid media. The first stage culturing is carried out in a liquid medium conventionally used for the tissue culture of plants, which contains indispensably inorganic substances and carbon sources and, additionally, phytohormones, vitamins and/or amino acids. While, the second stage culturing is carried out in another liquid medium, of which the concentration of at least one of the constituents is substantially varied, namely decreased or increased, from the first stage liquid medium. According to such two stage liquid medium cultures, the productivity of the second metabolites, such as shikonin, berberine and nicotine, are significantly increased, even though the rate of cell growth in the second stage is relatively low.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.