Production of complementary DNA representing RNA viral sequences by recombinant DNA methods and uses therefor
US4719177A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 12, 1981 |
| Grant date | Jan 12, 1988 |
| Priority date | — |
| Expiry date | Nov 12, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for producing RNA viral cDNA, such as poliovirus ds cDNA, products thereof, and uses thereof, are described. Poliovirus cDNA is produced, for example, by reverse transcribing poliovirus RNA and subsequently inserting the poliovirus cDNA into bacterial plasmids by genetic-engineering techniques. Transformed bacteria are then cloned and cultured to produce replicated chimeric plasmids containing the cDNA poliovirus. Such poliovirus cDNA is useful in assaying for the presence of poliovirus and in the production of antibodies against poliovirus. It has also been found that full-length poliovirus cDNA is infectious, which means it can be employed in producing altered virus particles for vaccines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.