Methods and compositions for detection of Legionnaires' disease
US4722891A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 20, 1984 |
| Grant date | Feb 2, 1988 |
| Priority date | — |
| Expiry date | Jun 20, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Recombinant E. coli clones which cell surface-express Legionella pneumophila antigens, a method for utilizing these clones for the detection of Legionella antibodies in a clinical sample, and a method for isolation of mono-specific anti-Legionella antibodies are disclosed. The recombinant clones are produced by ligating fragmented Legionella DNA to pBR322 which is then used to transform the appropriate E. coli host. Clones that cell surface-express individual Legionella antigens are selected by screening cellularly intact clones using anti-Legionella antibodies to probe for cell surface expression. An enzyme-linked immunosorbant assay (ELISA) is disclosed which utilizes the Legionella antigen-expressing clones to detect anti-Legionella antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.