Method of measuring specific binding reaction with the aid of polarized light beam and magnetic field
US4725140A · kind A · utility
Assignees
Inventor
Key dates
| Filing date | Oct 30, 1986 |
| Grant date | Feb 16, 1988 |
| Priority date | — |
| Expiry date | Oct 30, 2006 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/00534
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A coherent light beam is made incident upon a cell via a polarizer. In the cell is contained a reaction liquid consisting of fine magnetic particles having an antibody coated thereon and a sample containing an antigen which is specifically reacted with the antibody on the particles. The particles are rotated in the reaction liquid by means of alternating magnetic fields having a frequency f.sub.0 and generated by coils arranged beside the cell. Light scattered by the particles is made incident upon a photodetector via a polarizer whose polarization plane is perpendicular to that of the analyzer. An output signal from the photodetector is synchronously detected by means of a reference signal having a frequency 2f.sub.0. Then a synchronously detected output signal represents an amount of the antigen contained in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.