High performance electrophoretic mobilization of isoelectrically focused protein zones
US4725343A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 15, 1985 |
| Grant date | Feb 16, 1988 |
| Priority date | — |
| Expiry date | Oct 15, 2005 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/4473
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Protein zones which have been isoelectrically focused in a separation medium are mobilized for analytical or preparative purposes by changing the anolyte, catholyte or both to provide these two solutions with pH's varying in the same direction from the isoelectric points of each of said protein zones, then applying a voltage between them. The focused protein zones then elute as a unit from the separation medium while substantially maintaining their same positions relative to each other without hydrodynamic flow through the medium itself. Mobilization is alternatively achieved by hydrodynamic flow, for instance by pumping the focused protein zones with voltage maintained to suppress zone distortion (due to the hydrodynamic flow) and zone broadening (due to diffusion).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.