Preparations for use in solid phase immunoassays comprising monoclonal antibodies covalently embedded in their immobilized hybridoma cells

US4727023A · kind A · utility

Assignee

• I.D.L. International Diagnostic Laboratories Limited · IL

Inventors

• Lynn Wang · Jerusalem, IL
• Michael Inbar · Rehovot, IL

Key dates

• Filing date: Sep 9, 1985
• Grant date: Feb 23, 1988
• Expiry date: Sep 9, 2005

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/804
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A reagent for use in solid phase immunoassay diagnostics comprises a matrix of non-active hybridoma cells embedded with its self-produced, covalently bound, actively presented monoclonal antibodies. The solid phase reagent according to the invention is prepared by incubating in vitro a culture medium containing active hybridoma cells capable of producing monoclonal antibodies, allowing the formation of antibodies to proceed, separating and washing said cells, resuspending the cells in a buffer solution, adding to the resulting suspension an inactivator substance capable of converting active hybridoma cells into the non-active state.