Assay kit and method for the determination of antibody class and subclass
US4727037A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 15, 1984 |
| Grant date | Feb 23, 1988 |
| Priority date | — |
| Expiry date | Feb 15, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/81
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of rapid determination of the isotype class for a panel of monoclonal antibodies is described. The assay comprises adsorbing on a solid support medium antibodies directed to specific immunoglobulin heavy and light chains. Once such isotype-specific antibodies are bound to the nitrocellulose paper, the treated strips can be incubated with the monoclonal antibody of interest. Upon formation of a complex between the specific iso-type antisera and the monoclonal antibody, the complex is visualized by reaction with a chromogenic substance. In the preferred embodiment of the invention, the treated nitrocellulose strips are stored in kit form. Using these prepared strips, the isotyping assay can be performed in less than two hours with a minimum of technical manipulation and expenditure of reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.