Method for stabilizing recombinant DNA molecules and enhancing the expression thereof in strains of Bacillus
US4731327A · kind A · utility
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17Claims
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Key dates
| Filing date | Aug 18, 1986 |
| Grant date | Mar 15, 1988 |
| Priority date | — |
| Expiry date | Aug 18, 2006 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/67
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A recombinant DNA molecule, a portion of which includes a vector whose replication is initiated independently of the temperature-sensitive mutant gene of a temperature-sensitive mutant strain of the genus Bacillus as host is introduced into the host. The host is then cultured at a temperature which does not completely inhibit chromosomal DNA replication. By this process, the recombinant DNA molecule can be stabilized in the host and its expression can be enhanced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.