Purification method for alpha interferon
US4732683A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 2, 1986 |
| Grant date | Mar 22, 1988 |
| Priority date | — |
| Expiry date | Dec 2, 2006 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/56
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the purification of human interferon alpha involving a three-step chromatography sequence under highly dissociating conditions is disclosed. The purification method involves a first step of loading crude interferon alpha onto a glass sorbent column to adsorb interferon alpha. The adsorbed interferon alpha is then eluted from the glass sorbent using a hydrophobic electrolyte solution comprising tetramethyl ammonium chloride, NaCl and propylene glycol. Thereafter, in a second step, the eluted interferon is subjected to SEPHACRYL S-200 molecular sieving chromatography under highly dissociating conditions and the fractions corresponding to molecular weights ranging from about 10,000 to about 40,000 are collected. In a third step, the collected fractions are then passed thorugh a column Zn++ chelating resin. The interferon alpha passes through unadsorbed to the Zn++ chelating resin. The resulting solution is a highly purified interferon alpha which preserves the apparently native isospecies compositions. Also disclosed is a method to enhance overall interferon alpha recovery which involves treating crude or partially purified interferon alpha solutions with a non-ionic det…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.