Process for determining the perkallikrein content and the partial thromboplastin time of a plasma sample

US4732860A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Knut Bartl · Weilheim in Oberbayern, DE
• Helmut Lill · Wielenbach, DE

Key dates

• Filing date: Jan 24, 1986
• Grant date: Mar 22, 1988
• Expiry date: Jan 24, 2006

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/96455
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a process for the photometric determination of prekallikrein in plasma, wherein plasma is incubated with a surface activator, the extinction obtained is measured, a chromogenic thrombin substrate is then added thereto, the optically determinable group liberated therefrom is measured at short time intervals or continuously and the gradient of the linear part of the curve obtained by plotting the time against the extinction is determined as a measure of the prekallikrein content. The partial thromboplastin time of plasma is simultaneously determined by carrying out the incubation in the presence of calcium ions and a phospholipid and measuring the time it takes the extinction to reach a predetermined level.