Process for purifying recombinant proteins, and products thereof
US4734362A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 25, 1986 |
| Grant date | Mar 29, 1988 |
| Priority date | — |
| Expiry date | Sep 25, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/826
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a process for recovering recombinant protein from refractile inclusion bodies of microorganisms which comprise disrupting cells, recovering refractile bodies containing recombinant protein, solubilizing the refractile bodies with a denaturant, protecting sulfhydryl groups of recombinant proteins, converting cationic amino groups of recombinant proteins to anionic carboxylic acid by derivatization with organic cyclic acid anhydrides, and recovering recombinant protein derivatives. The present invention also relates to the production of solid supports coated with recombinant protein recovered by the process of the invention, and the use of said devices for the detection and quantification of antibodies to said recombinant proteins in body fluids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.