Large scale production of DNA probes
US4734363A · kind A · utility
31Cited by
5References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 27, 1984 |
| Grant date | Mar 29, 1988 |
| Priority date | — |
| Expiry date | Nov 27, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6876
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for production of a single strand of a nucleic acid comprising covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said polynucleotide with an oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized polynucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the extended oligonucleotide. The product can be used for making analytical probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.