Method and kit for detecting polyriboadenosine segments and messenger RNA

US4735897A · kind A · utility

Assignees

• ALLIED CONSULTING, INC. · US
• Genetics Institute, LLC · US

Inventors

• Calvin P. H. Vary · Frye Island, US
• Steven E. Diamond · Springfield, US
• Neil M. Wolfman · Brookline, US
• Astrid P. Koudelka · Somerville, US

Key dates

• Filing date: May 2, 1985
• Grant date: Apr 5, 1988
• Expiry date: May 2, 2005

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/975
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

RNA such as messenger RNA is digested to nucleotide phosphates including AMP or ADP. The ATP or a byproduct of the phosphorylation, e.g., pyruvate, is detected. Exemplary enzymes used (with appropriate co-reactants and co-factors) are: (1) polynucleotide phosphorylase, pyruvate kinase and luciferase, or (2) phosphodiesterase (or RNase), myokinase, pyruvate kinase and luciferase. The phosphorylation to ATP (e.g., with pyruvate kinase) is preferably coupled with the previous (reversible) enzymatic step.