Stabilized fluorescent rare earth labels and labeled physiologically reactive species
US4735907A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 18, 1985 |
| Grant date | Apr 5, 1988 |
| Priority date | — |
| Expiry date | Mar 18, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2982
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Improved fluorescent labels comprise a fluorescent rare earth chelate incorporated into a polymeric particle derived from a loadable latex. The polymer comprises: (a) from about 50 to about 96 weight percent of recurring units derived from a hydrophobic ethylenically unsaturated polymerizable monomer, (b) from about 2 to about 30 weight percent of recurring units derived from a nonionic hydrophilic ethylenically unsaturated polymerizable monomer, and (c) from about 2 to about 20 weight percent of recurring units derived from an anionic ethylenically unsaturated polymerizable monomer containing at least one solubilizing group. The labels of this invention have greatly improved stability in aqueous solutions and do not prematurely agglomerate during storage. They can be attached to any of a variety of physiologically reactive species to provide labeled species, and are particularly useful in specific binding assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.