Patent · US Expired

Trans-acting transcriptional factors

US4738922A · kind A · utility

55Cited by
1References
26Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 25, 1984
Grant dateApr 19, 1988
Priority date
Expiry dateMay 25, 2004

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2740/16322
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

This invention describes the discovery of a novel phenomena in retrovirus transcription, namely transcriptional trans-activation. Described herein are novel trans-acting factors which may be employed to enhance the production of heterologous genes. Described is a novel trans-acting directing gene, designated herein as the "luk" gene and the 35,000 to 45,000, more specifically about 42,000 dalton molecular weight protein encoded thereby. The present invention demonstrates the LTR elements of HTLV can function as transcriptional promoters for heterologous genes on both unintegrated and integrated DNA. In general, the HTLV-1 LTR is a stronger promoter than is the HLTV-II LTR in its requirements for cellular and/or viral trans-acting factors in order to function efficiently. HTLV infection results in the production of trans-acting factors that dramatically increase the rate of HTLV LTR-promoted transcription.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.