Method for derivitization of polynucleotides
US4739044A · kind A · utility
196Cited by
3References
5Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 13, 1985 |
| Grant date | Apr 19, 1988 |
| Priority date | — |
| Expiry date | Jun 13, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method for the preparation of a 3' end functionalized polynucleotide is disclosed. An amine-functionalized solid phase support is treated sequentially with an anhydride, then with an .omega.-hydroxylamine. A polynucleotide is chemically synthesized on the treated support and is subsequently cleaved therefrom by hydrolysis of the amide bonds. A polynucleotide having a 3' free primary amine is recovered for use in hybridization assays and other uses.
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