Patent · US Expired

Chromatographic column

US4740306A · kind A · utility

18Cited by

11References

13Claims

0Family size

Assignee

Temple University-Of The Commonwealth System of Higher Education · US

Inventors

Gerald Litwack · Via Enrico Fermi, US
Ted M. Kirsch · Philadelphia, US

Key dates

Filing date	Mar 17, 1986
Grant date	Apr 26, 1988
Priority date	—
Expiry date	Mar 17, 2006

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/815
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A chromatographic column and process for isolating or purifying steroid hormone or cell membrane receptors using the column are provided in which the column contains at least three resin layers between the inlet and the outlet ends of the column, the layer closest the inlet being a strong cationic exchange resin, the middle layer being a matrix containing a triazine dye that will bind proteases and proteins with dinucleotide fold conformations, and the layer closest the outlet being a weak anionic exchange resin, the ratio of the volumes of the resin layers being 1:2:1.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.