Vectors and methods for transformation of eucaryotic cells
US4740461A · kind A · utility
213Cited by
4References
12Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 27, 1983 |
| Grant date | Apr 26, 1988 |
| Priority date | — |
| Expiry date | Dec 27, 2003 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21069
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Eucaryotic cells cotransformed with product and selection genes yield considerably greater quantities of product after a novel subcloning strategy is employed: Transformants are identified for product yield, cultured under selection pressure and the progeny screened for product yield. Novel transformation vectors contain directly ligated selection and product genes and/or eucaryotic promoters.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.