Monoclonal antibodies useful in the identification of microorganisms causing periodontal disease
US4741999A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 26, 1986 |
| Grant date | May 3, 1988 |
| Priority date | — |
| Expiry date | Feb 26, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Actinobacillus actinomycetemcomitans has frequently been implicated in juvenile periodontitis. The present monoclonal antibodies are specific to Actinobacillus actinomycetemcomitans. The present monoclonal antibodies are typically employed as reagents which include an inert carrier, preferably a liquid, such as buffered saline solution and a preservative. The carrier compositions are suitably selected to provide for the proper dispersal of bacteria, and to preserve the integrity of antigens and supplemental structures. The selection of the proper carrier is especially important in the detection in mixtures which include bacteria which produce large amounts of autolyltic enzymes such as B. gingivalis. The monoclonal antibodies of the present invention are useful in clinical testing and differentiating antigens in the gingival or subgingival sera. The method of testing and differentiating involves the steps of contacting a sample of the bacterial flora from a lesion or other site with a measured amount of monoclonal antibody specific to a single antigen site on Actinobacillus actinomycetemcomitans and subsequently measuring the number of antibody-antigen complexes formed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.