Method for producing enzymatically active eucaryotic sod in bacteria
US4742004A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 27, 1984 |
| Grant date | May 3, 1988 |
| Priority date | — |
| Expiry date | Aug 27, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y115/01001
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of producing enzymatically active eucaryotic superoxide dismutase or an analog thereof in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase or analog thereof comprising maintaining the bacterial cell under suitable conditions and in a suitable production medium. The production medium is supplemented with an amount of Cu++ so that the concentration of Cu++ in the medium is greater than about about 2 ppm. The invention also concerns methods of recovering purified enzymatically active eucaryotic superoxide dismutase or analogs from bacterial cells producing the superoxide dismutase or analog.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.