Purification of hepatitis pre-S antigens by polymerized serum albumin affinity binding
US4742158A · kind A · utility
15Cited by
5References
9Claims
0Family size
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Key dates
| Filing date | Apr 14, 1987 |
| Grant date | May 3, 1988 |
| Priority date | — |
| Expiry date | Apr 14, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/826
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant pre-S-HBsAg is purified by a rapid and efficient two step chromatographic process. Yeast cells expressing recobinant pre-S-HBsAg are disrupted, the cell contents are clarified and separated by polymerized human serum albumin affinity chromatography. The pre-S-HBsAg is further purified by hydrophobic interaction chromatography using butyl agarose. This process results in pre-S-HBsAg that is greater than 90% pure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.