Digitalis antibodies, process for the preparation thereof and the use thereof for the therapy of digitalis intoxications

US4742159A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Hans-Georg Batz · Tutzing, DE
• Herbert Jungfer · Starnberg, DE
• Helmut Lenz · Tutzing, DE
• Albert Roder · Seeshaupt, DE

Key dates

• Filing date: Dec 19, 1983
• Grant date: May 3, 1988
• Expiry date: Dec 19, 2003

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/866
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides a process for the preparation of digitalis antibodies in which appropriate mammals are immunized with a digoxin bound to protein, the animal serum is obtained, the immune globulin-containing protein fraction is separated in known manner, the immunologically-active globulins are adsorbed on an immunologically-active column and separated from the other proteins, the antibodies are again eluted from the column and the Fab fractions are split with papain and purified, wherein, as immunologically-active adsorbent, there is used an inorganic matrix of large surface area to which digitoxin aldehyde is bound via a spacer which cannot be split with papain and the splitting off of the Fab fraction from the antibodies is carried out on the matrix. The present invention also provides digitalis antibodies obtained by this process, which antibodies can be used for the therapy of digitalis intoxications and for preparing immunological reagents for the determination of digitalis glycosides.