Method for forestalling the hook effect in a multi-ligand immunoassay system
US4743542A · kind A · utility
44Cited by
3References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 11, 1985 |
| Grant date | May 10, 1988 |
| Priority date | — |
| Expiry date | Apr 11, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/818
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is provided for substantially increasing the dynamic range of an immunoassay and forestalling the hook effect observed with very large ligand concentrations in aqueous samples. The method comprises the addition of ligand binding partners which do not have labels associated therewith to compete with labeled ligand binding partners thereby effectively eliminating the presence of the excess ligand in the sample solution. The conversant method for ligand binding partner immunoassays is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.