Plant cell microinjection technique
US4743548A · kind A · utility
64Cited by
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8Claims
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Key dates
| Filing date | Aug 1, 1985 |
| Grant date | May 10, 1988 |
| Priority date | — |
| Expiry date | Aug 1, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Microinjection techniques for plant protoplasts utilize a holding pipette for immobilizing the protoplast while an injection pipette is utilized to inject the macromolecule. In order to manipulate the protoplasts without damage, the protoplasts are cultured for from about 1 to 5 days before the injection is performed to allow for partial regeneration of the cell wall. It was found that injection through the partially regenerated cell wall could still be accomplished and particular compartments of the cell could be targeted. The methods are particularly useful for transformation of plant protoplasts with exogenous genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.