Process for producing human epidermal growth factor and analogs thereof
US4743679A · kind A · utility
61Cited by
2References
6Claims
0Family size
Assignee
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Key dates
| Filing date | Feb 24, 1986 |
| Grant date | May 10, 1988 |
| Priority date | — |
| Expiry date | Feb 24, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a process for producing a fusion protein comprising epidermal growth factor (EGF) attached through a Glu-residue to a leader. Treatment of the fusion protein with a Staphylococcus aureus V8 protease specific for cleaving peptides at a Glu-linkage produces large amounts of the 53 amino acid EGF sequence. The treatment conditions optimize production of the 53 and 51 amino acid EGFs relative to shorter analogs, and take full advantage of the selectivity of the protease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.