Immunoassay and immunometric assay of free ligand concentrations in biological fluids
US4745072A · kind A · utility
Inventors
Key dates
| Filing date | Feb 20, 1985 |
| Grant date | May 17, 1988 |
| Priority date | — |
| Expiry date | Feb 20, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/817
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of measuring the concentration of a free ligand in a biological fluid containing the free ligand and ligand bound to endogenous binding agent, by the steps of PA0 (a) mixing a sample of the fluid with an analogue of the ligand, a specific binder with which the free ligand and the ligand analogue bind, and an exogenous binding agent which binds the ligand analogue but not the ligand, either the ligand analogue or the specific binder being labelled, PA0 (b) incubating the resulting mixture, PA0 (c) determining either the amount of the labelled analogue bound or the amount of labelled specific binder bound, or not bound, to the ligand analogue, and PA0 (d) correlating the determined amount to the amount of free ligand present in the sample. The method is useful to measure concentration of free thyroid hormones and other hormones in body fluids, employing antibodies specific to the ligand analogue as the exogenous binding agents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.