Method of performing assay for analyte in liquid medium
US4745077A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 17, 1985 |
| Grant date | May 17, 1988 |
| Priority date | — |
| Expiry date | Jan 17, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/809
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention concerns assays, such a immunoassays or two-site immunometric assays, performed using a labelled reagent and another reagent bound to magnetically attractable particles which are suspendable but insoluble in a liquid assay medium. After the labelled reagent has become partitioned between the liquid phase and the particles, in proportions which depend on the concentration of an analyte in a sample, the liquid phase is removed. Then the particles are re-suspended in another liquid medium, and the concentration of label observed. The method is particularly suitable for fluorescent and chemiluminescent' label systems, and can conveniently be performed in microtiter plates in which the wells are optically screened from one another, the observations being made from above or below the wells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.