Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
US4745183A · kind A · utility
5Cited by
1References
8Claims
0Family size
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Key dates
| Filing date | Dec 3, 1984 |
| Grant date | May 17, 1988 |
| Priority date | — |
| Expiry date | Dec 3, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/808
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel method is disclosed for the purification of monoclonal antibodies from antibodies of the same isotype but different light chain composition which are frequently present in the fluids in which such antibodies are generated. According to the method, the mixture is placed in a hydroxylapatite chromatography column and eluted with a buffer solution in a concentration gradient elution. With the appropriate optimization of system parameters, a separation of antibodies effective for both analytical and preparative purposes is obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.