Method for determining lipid bound sialic acid in plasma
US4748128A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 18, 1985 |
| Grant date | May 31, 1988 |
| Priority date | — |
| Expiry date | Mar 18, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/255
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The amount of lipid bound sialic acid in a blood plasma or serum sample may be determined by a method which may be automated involving the following steps: diluting the sample with distilled water; mixing and then cooling the diluted sample; adding a mixture of a chlorinated lower alkyl hydrocarbon and a lower alkyl alcohol; mixing, diluting with water and then treating by mixing further and centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a mixture of a protein precipitating agent and adsorbing material, mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in distilled water and determining the amount of lipid bound sialic acid present.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.