Process for the purification of gamma interferon

US4751078A · kind A · utility

Inventors

• Tattanahalli L. Nagabhushan · Parsippany, US
• Paul P. Trotta · Rutherford, US
• Hung V. Le · Rockaway, US
• Gail F. Seelig · Watchung, US
• Robert A. Kosecki · Parsippany, US

Key dates

• Filing date: Jul 3, 1985
• Grant date: Jun 14, 1988
• Expiry date: Jul 3, 2005

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/811
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process is disclosed for purifying gamma interferon from various contaminants resulting from disruption of the cell in which the interferon was produced. The process provides for sequential removal of (a) nucleic acids, (b) negatively charged contaminating proteins, (c) positively charged contaminating proteins and (d) low and high molecular weight impurities.