Methods and kits for performing nucleic acid hybridization assays
US4751177A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 13, 1985 |
| Grant date | Jun 14, 1988 |
| Priority date | — |
| Expiry date | Jun 13, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/224
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the isolation and quantitative detection of a selected single-stranded target polynucleotide from solution. The target polynucleotide is hybridized in solution to a single-stranded mediator polynucleotide, a probe polynucleotide, and an immobilized polynucleotide sequence. The sequence of the mediator polynucleotide comprises a first sequence complementary to a first portion of the target polynucleotide sequence and a second nucleotide sequence complementary to a portion of a single-stranded immobilized polynucleotide sequence. The probe polynucleotide, which carries a detectable label, is complementary to a second portion of the necleotide sequence of the target. The immobilized polynucleotide is immobilized by attachment to a solid support, and, through hybridization to the mediator polynucleotide, functions to immobilize the entire immobilized polynucleotide/target polynucleotide/probe polynucleotide "sandwich".
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.