Method and reagents for differential determination of four populations of leukocytes in blood
US4751179A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 31, 1984 |
| Grant date | Jun 14, 1988 |
| Priority date | — |
| Expiry date | May 31, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/107497
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention describes a reagent system, including saponin in a lysing reagent and a rapidly active cross-linking agent such as glutaraldehyde as a fixing reagent, which reproducably affects whole blood to cause the red blood cells to stromatolyze and modifies the leukocytes to generate data to define four distinct clusters for detection and classification by flow analysis instrumentation. The clusters represent the four major leukocyte types found in blood: lymphocytes, monocytes, neutrophils and eosinophils, thus providing a method of leukocyte differential analysis. The parameters used for the leukocyte classification include combinations of two or more of DC (Coulter) volume, high frequency (RF) size, opacity (RF size/DC volume), light scatter at various angular ranges, and fluorescence at various wavelengths of illumination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.