Method for assaying proteases with tagged proteinaceous inhibitors
US4753875A · kind A · utility
Inventor
Key dates
| Filing date | Jul 26, 1985 |
| Grant date | Jun 28, 1988 |
| Priority date | — |
| Expiry date | Jul 26, 2005 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/96472
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A new and useful method for assaying proteases, in which an excess of tagged proteinaceous inhibitor of a protease is mixed in solution with a sample containing the protease to form a mixture of a tagged inhibitor-protease complex and free tagged inhibitor. The complex is separated from the tagged inhibitor and the tag measured. Alternatively, anti-protease antibody may be added to bind the complex so that assay specificity and/or separation is enhanced. The method of this invention is applicable to assaying minute quantities of clinically significant proteases e.g. renin, kallikrein, thrombin occurring in serum, plasma, urine and other tissues.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.