Assay systems using more than one enzyme
US4758323A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 7, 1984 |
| Grant date | Jul 19, 1988 |
| Priority date | — |
| Expiry date | May 7, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
This specification discloses methods of detection or measurement of an enzyme or of its specific substrate, and sensors used in such methods. The present invention is concerned with a multi-enzyme system, and the specification discloses, as one aspect of the invention a method of assay in which an electrode poised at a suitable potential is contacted with a system comprising a first enzyme, a cofactor linked with said enzyme and a mediator compound which transfers charge to the electrode from the first enzyme when its electrical state is changed by reaction of cofactor material. The cofactor may be NAD, NADP (both collectively referred to herein as NAD(P)), cAMP, ATP, GTP, TTP, or CTP. The specification particularly illustrates a method of assay in which an electrode (1) poised at a suitable potential is contacted with a system comprising a first enzyme E.sub.1 a nicotinamide adenine dinucleotide compound N linked with said enzyme E.sub.1 and a mediator compound F which transfers charge to the electrodes from the first enzyme when its electrical state is changed by a NAD(P) NAD(P)H reaction. The NAD compound may act as a "bridge" between the said enzyme/mediator system and further …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.