Monoclonal antibodies with specificity for crosslinked fibrin derivatives and assay for said derivatives
US4758524A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 15, 1984 |
| Grant date | Jul 19, 1988 |
| Priority date | — |
| Expiry date | Mar 15, 2004 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/75
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a method of a preparation of monoclonal antibody derived from a crosslinked fibrin derivative including the steps of: PA0 (i) obtaining a crosslinked fibrin derivative or extract containing same; and PA0 (ii) forming an antibody to said derivative or extract by cloning antibody producing cells from an animal having been administered thereto said derivative or extract. A screening assay for use in the abovementioned method which includes the steps of: PA0 (A) coating a surface with an antigen selected from a PA1 (i) crosslinked fibrin derivative; PA1 (ii) extract containing said derivative; or PA1 (iii) fibrinogen degradation product. PA0 (B) contacting said antigen with a monoclonal antibody derived from crosslinked fibrin derivative; and PA0 (C) subjecting the complex formed in step (A) to a signal amplification step and an assay procedure for detection of the presence of a crosslinked fibrin derivative in an animal body fluid including the steps of: PA1 (i) contacting a monoclonal antibody prepared from a crosslinked fibrin derivative with a fluid sample suspected of containing an antigen derived from a crosslinked fibrin derivative or comprising a crossl…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.