Rapid method for the detection of beta-lactamase in body fluids
US4760018A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 17, 1985 |
| Grant date | Jul 26, 1988 |
| Priority date | — |
| Expiry date | Jun 17, 2005 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2415/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A rapid method for the detection of beta-lactamase in body fluids which method comprises contacting a sample of a body fluid with charcoal, removing the charcoal and recovering an essentially charcoal free supernatant, adding a labeled beta-lactam compound such as C.sup.14 penicillin to the supernatant for a defined period of time, adding a antibiotic sensitive microorganism, incubating and thereafter removing the microorganism cells from the incubation broth, measuring the amount of C.sup.14 bound-labeled penicillin and comparing the measured amount with a controlled sample or standard data or graph to determine the amount of any beta-lactamase in the body fluid sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.