Size-exclusion chromatography system for macromolecular interaction analysis
US4762617A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 15, 1987 |
| Grant date | Aug 9, 1988 |
| Priority date | — |
| Expiry date | Jan 15, 2007 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/89
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A low pressure, microcomputer controlled system employing high performance liquid chromatography (HPLC) allows for precise analysis of the interaction of two reversibly associating macromolecules such as proteins. Since a macromolecular complex migrates faster than its components during size-exclusion chromatography, the difference between the elution profile of a mixture of two macromolecules and the summation of the elution profiles of the two components provides a quantifiable indication of the degree of molecular interaction. This delta profile is used to qualitatively reveal the presence or absence of significant interaction or to rank the relative degree of interaction in comparing samples and, in combination with a computer simulation, is further used to quantify the magnitude of the interaction in an arrangement wherein a microcomputer is coupled to analytical instrumentation in a novel manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.