Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine phosphate detection
US4767699A · kind A · utility
32Cited by
8References
27Claims
0Family size
Assignee
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Key dates
| Filing date | May 2, 1985 |
| Grant date | Aug 30, 1988 |
| Priority date | — |
| Expiry date | May 2, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A reagent complex is disclosed containing (1) a probe polynucleotide having a target binding region complementary to a target nucleotide sequence and (2) a signal strand polynucleotide bound by base pairing to a portion of the target binding region. The signal strand is displaced from the reagent complex by the target nucleotide sequence and separated from intact reagent complexes. A digestable ribonucleotide segment of the displaced signal strand, such as a 3' , terminal segment, is digested to ribonucleotide phosphates, which are phosphorylated to ATP. The ribonucleotide phosphates or ATP are detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.