Immobilization of enzymes with a water-soluble glycidyl ether

US4767707A · kind A · utility

Assignee

• BASF SE

Inventors

• Stefan Marcinowski · Ludwigshafen, DE
• Axel Sanner · Bischofswerda, DE
• Chung-Ji Tschang · Bad Dürkheim, DE
• Wolfgang Ladner
• Norbert Greif · Worms, DE

Key dates

• Filing date: Jul 25, 1986
• Grant date: Aug 30, 1988
• Expiry date: Jul 25, 2006

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N11/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Enzymes are immobilized by mixing the following components with one another in aqueous solution: PA0 (a) an enzyme or a dispersed microorganism, PA0 (b) a water-soluble glycidyl ether, prepared by subjecting from 1 to 30 equivalents of an epoxide of the formula I ##STR1## where R.sup.1, R.sup.2 and R.sup.3 are each hydrogen, methyl or ethyl, to an addition reaction with 1 equivalent (based on OH groups) of a polyfunctional alcohol of 2 to 6 carbon atoms or of a mono- or disaccharide to produce an adduct, reacting the adduct with epichlorohydrin and, if required, then carrying out cyclization to give the epoxide, and PA0 (c) a water-soluble amine possessing two or more NH groups, if necessary in a partially neutralized form, and allowing the mixture thus obtained to gel.