Expression linkers
US4769326A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 12, 1984 |
| Grant date | Sep 6, 1988 |
| Priority date | — |
| Expiry date | Apr 12, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/31
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is based upon a general principle of providing specific oligonucleotide segments ("linkers", herein) to be attached in sequence to a cloned DNA coding segment. The linkers of the present invention confer desired functional properties on the expression of the protein coded by the coding sequence. Using linkers of the present invention, the desired protein may be expressed either as a fusion or non-fusion protein. A linker coding for an additional sequence of amino acids may be attached, the sequence being chosen to provide properties exploitable in a simplified purification process. A linker coding for an amino acid sequence of the extended specific cleavage site of a proteolytic enzyme is provided, as well as specific cleavage linkers for simpler specific cleavage sites.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.