Method for determination of enzyme activity
US4772553A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 17, 1986 |
| Grant date | Sep 20, 1988 |
| Priority date | — |
| Expiry date | Jun 17, 2006 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/976
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a method for determining an enzyme-, an enzyme inhibitor-, an enzyme activator-, or zymogen-activity, which comprises allowing the enzyme to react with a substrate of the formula ##STR1## (wherein R.sub.1 represents an amino acid of an oligopeptide combined in the form of ester through the C-terminal carboxyl group of said amino acid or oligopeptide and R.sub.2 represents a hydrogen or bromine atom) to hydrolyze the substrate, then allowing the hydrolysis product of the formula ##STR2## to react with a FR-ITR salt [Fast Red-ITR salt (N,N'-diethyl-4-methoxymetanilamide diazonium salt)] to form a pigment, and determining the pigment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.