Polypeptide cartilage-inducing factors found in bone
US4774322A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 10, 1987 |
| Grant date | Sep 27, 1988 |
| Priority date | — |
| Expiry date | Dec 10, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/12
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.