Method of determining sugar content of tobacco using a discrete analyzer
US4777131A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 13, 1987 |
| Grant date | Oct 11, 1988 |
| Priority date | — |
| Expiry date | Jan 13, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/203332
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of determining in a discrete analyzer the total sugars content of a tobacco sample containing sucrose, glucose and fructose, comprises the steps of, converting the sucrose to glucose and fructose, enzymatically converting the glucose and fructose to glucose-6-phosphate in the presence of hexokinase, reacting the glucose-6-phosphate with nicotinamide-adenine dinucleotide in the presence of glucose-6-phosphate dehydrogenase to give reduced nicotinamide-adenine dinucleotide, the amount of reduced nicotinamide-adenine dinucleotide produced being directly proportional to the total sugars content of the sample, measuring the concentration of reduced nicotinamide-adenine dinucleotide in the sample, and generating a signal indicative of the concentration of reduced nicotinamide-adenine dinucleotide and hence of the total sugars content of the sample. Deactivation of enzymes by any cyanogen bromide that may be present is prevented by the addition of dithioerythritol to the reaction mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.