Proteinaceous substance showing antitumorous action and its preparing method
US4777241A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 21, 1986 |
| Grant date | Oct 11, 1988 |
| Priority date | — |
| Expiry date | Jul 21, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/144
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A proteinaceous substance having the following biochemical characteristics: PA0 (A) a strong cytolytic activity against mouse tumor cells L-929, but not cytotoxic activity for normal cells such as human fetus fibroblasts, human adult skin fibroblasts and chinese hamster fibroblasts (Don, V-79) and therefore a high tumor specificity: and PA0 (B) a strong anti-tumor and immunogenic activity against Meth-A sarcoma. When said substance is injected intravenously to the syngenic BALB/C mice inoculated with Meth-A sarcoma at a quantity of more than 72 units head, not only hemorrhagic necrosis, but also complete degradation is observed. Thereafter, even if it is attempted again to transplant the Meth-A sarcoma cells under the skin of Mice cured completely, successful plantation is not observed because of an acquired immunity: PA0 (C) the substance has no pyrogenetic action after intravenous injection to rabbits: PA0 (D) a molecular weight of 45,000.+-.5,000 (determined by the gel filtration method with Sephacryl S-200): and PA0 (E) an isoelectric point pI of 4.8+0.3: and a method for its manufacture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.